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KMID : 0380220070400020189
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 2 p.189 ~ p.195
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Transduced PEP-1-Grb7 Fusion Protein Suppressed LPS-induced COX-2 Expression
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An Jae-Jin
Kim So-Young
Lee Sun-Hwa
Kim Dae-Won
Ryu Hea-Jin
Yeo Seung-Il
Jang Sang-Ho
Kwon Hyung-Joo
Kim Tae-Yoon
Lee Sang-Chul
Poo Ha-Ryoung
Cho Sung-Woo
Lee Kil-Soo
Park Jin-Seu
Eum Won-Sik
Choi Soo-Young
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Abstract
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Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.
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KEYWORD
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Atopic dermatitis (AD), COX-2, PEP-1-Grb7, Protein therapy, Protein transduction
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